The complete DNA sequence of the mitochondrial genome of a "living fossil," the coelacanth (Latimeria chalumnae).
نویسندگان
چکیده
The complete nucleotide sequence of the 16,407-bp mitochondrial genome of the coelacanth (Latimeria chalumnae) was determined. The coelacanth mitochondrial genome order is identical to the consensus vertebrate gene order which is also found in all ray-finned fishes, the lungfish, and most tetrapods. Base composition and codon usage also conform to typical vertebrate patterns. The entire mitochondrial genome was PCR-amplified with 24 sets of primers that are expected to amplify homologous regions in other related vertebrate species. Analyses of the control region of the coelacanth mitochondrial genome revealed the existence of four 22-bp tandem repeats close to its 3' end. The phylogenetic analyses of a large data set combining genes coding for rRNAs, tRNAs, and proteins (16,140 characters) confirmed the phylogenetic position of the coelacanth as a lobe-finned fish; it is more closely related to tetrapods than to ray-finned fishes. However, different phylogenetic methods applied to this largest available molecular data set were unable to resolve unambiguously the relationship of the coelacanth to the two other groups of extant lobe-finned fishes, the lungfishes and the tetrapods. Maximum parsimony favored a lungfish/coelacanth or a lungfish/tetrapod sistergroup relationship depending on which transversion:transition weighting is assumed. Neighbor-joining and maximum likelihood supported a lungfish/tetrapod sistergroup relationship.
منابع مشابه
A white paper for sequencing the genome of a living fossil: the coelacanth, Latimeria chalumnae
متن کامل
Two living species of coelacanths?
During the period of September 1997 through July 1998, two coelacanth fishes were captured off Manado Tua Island, Sulawesi, Indonesia. These specimens were caught almost 10,000 km from the only other known population of living coelacanths, Latimeria chalumnae, near the Comores. The Indonesian fish was described recently as a new species, Latimeria menadoensis, based on morphological differentia...
متن کاملSearching for the closest living relative(s) of tetrapods through evolutionary analyses of mitochondrial and nuclear data.
The phylogenetic relationships of the African lungfish (Protopterus dolloi) and the coelacanth (Latimeria chalumnae) with respect to tetrapods were analyzed using complete mitochondrial genome DNA sequences. A lungfish + coelancanth clade was favored by maximum parsimony (although this result is dependent on which transition:transversion weights are applied), and a lungfish + tetrapod clade was...
متن کاملThe hypothesis that coelacanth is the closest living relative of tetrapods 3 was rejected based on three genome-scale approaches
21 2 1 Since its discovery of the " living fossil " in 1938, the coelacanth (Latimeria chalumnae) has generally 2 been considered to be the closest living relative of the land vertebrates, and this is still the prevailing 3 opinion in most general biology textbooks. However, the origin of tetrapods has been the subject of 4 intense debate for decades. The three principal hypothesis (lungfish-te...
متن کاملExtrapolating movement without retinal motion.
Comoran coelacanths are sluggish, nocturnal drift hunters that feed on other fish and have a low metabolic rate 6. During the day, they retreat to the still water of deep lava caves 1. The Indonesian sites would therefore seem to be unsuitable for sustaining a viable population of L. chalumnae. So far, four Indonesian coelacanths have been found in a relatively small area along the north Sulawe...
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ورودعنوان ژورنال:
- Genetics
دوره 146 3 شماره
صفحات -
تاریخ انتشار 1997